Interferon-γ Release Assay and Reverse Blot Hybridization Assay: Diagnostic Role in Cutaneous Tuberculosis

Diagnostic Utility of Interferon-γ Release Assay in Tuberculous Lymphadenitis

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...

The role of interferon-gamma release assay in tuberculosis control.

Tuberculosis is still one of the major global public health threats. Countries with low incidence must focus on exhausting the reservoir of future cases by preventing reactivation. Therefore, it is important to identify and effectively treat those individuals who have latent tuberculosis infection and who may develop active disease. The tuberculin skin test has been the standard for detection o...

Pleural effusions, eosinophilia and a positive interferon-γ release assay.

tive except for a 1-kg weight loss in the preceding 4 months. His social history was notable for living in South Korea his entire life until immigrating to the United States 10 years prior to his present illness, with no intercurrent travel since that time. On physical examination, his vital signs were notable for a temperature of 37.9 ° C with normal respiratory rate and pulse oximetry on room...

Interferon-Gamma Release Assay Performance in Pulmonary and Extrapulmonary Tuberculosis

BACKGROUND The diagnosis of tuberculosis remains difficult. This study aimed to assess performance of interferon-gamma release assay (IGRA) in diagnosis of active tuberculosis (ATB) with pulmonary and extrapulmonary involvements, and to determine the diagnostic role of IGRA (T-SPOT.TB) and tuberculin skin test (TST) in BCG-vaccinated population. METHODS AND FINDINGS Two hundred twenty-six ATB...